CheckLite™ CT
Characteristics of kit
CheckLite™ CT is a rapid detection kit for the presence of total coliforms, using a sensitive bioluminometric assay for beta-galactosidase in coliform bacteria.
- More rapid detection method than colorimetric assays or fluorescent assays.
- Detection for presence or absence of coliform bacteria (not quantitative)
Table. lncubation time required for detecting coliforms
| Strain | Incubation time |
| Escherichia coli IAM12119 Citrobacter freundii JCM1657 Enterobacter cloacae IAM12349 |
5h 6h 7h |
Procedure
Principle of Measurement
- CheckLite™ CT150 is an enzymatic presence-absence test for total coliforms relying on the detection of beta-galactosidase. The “Detection reagent CT” contains D-luciferin-O-beta-galacTOPyranoside (LuGal) as an indicator of beta-galactosidase activity, and luciferase.
- Luciferin produced from LuGal by beta-galactosidase can be detected as luminescence using luciferase. The amount of bioluminescence is direct proportion to the amount of beta-galactosidase from coliform bacteria in the sample culture.
- A highly sensitive bioluminometric assay of beta-galactosidase allows more rapid detection of coliforms than colorimetric assays.
CheckLite™ CT150 should be stored at 2-8 C in a refrigerator. DO NOT FREEZE.
For more information you can contact our office or you can refer to the relevant specification (pdf)
